1. This enzyme is a recombinant protein isolated by cloning the EcoliK12Uracil-N-Glycosylase gene in Escherichia coli, inducing its expression, and then purifying it three times through a column. Molecular weight 25.66 KDa.
2. Reaction condition: 50℃,2 min; reaction buffer: 20 mM Tris-HCl(pH: 8.0);1 mM EDTA;1 mM DTT.