PCR Series-Probe Method Quantitative PCR Premix（universal）
PCR Series-Probe Method Quantitative PCR Premix（universal）
PCR Series-Probe Method Quantitative PCR Premix（universal）

PCR Series-Probe Method Quantitative PCR Premix（universal）

Taq HS Probe qPCR Premix (Universal) is a 2× premixed liquid product for real-time quantitative PCR , developed based on unique two-component hot start Taq polymerase. This product already contains all fluorescence quantitative PCR components except for primers and sample DNA, which can reduce operational steps, shorten sampling time, and reduce the probability of contamination. It is suitable for TaqMan probe using cDNA or DNA as samples. 

  • Product Introduction
  • Product Features
  • FAQ/Experimental Cases
  • Manual Download
Article No.Product NameChinese NamespecificationsPrice（Yuan）
DQ00301Taq-HS Probe qPCR Premix (Universal)探针法定量PCR预混液（通用）2 ×，4 ml888

Taq HS Probe qPCR Premix (Universal) is a 2× premixed liquid product for real-time quantitative PCR , developed based on unique two-component hot start Taq polymerase. This product already contains all fluorescence quantitative PCR components except for primers and sample DNA, which can reduce operational steps, shorten sampling time, and reduce the probability of contamination. It is suitable for TaqMan probe using cDNA or DNA as samples. Due to the different designs of some brand models of fluorescence quantitative PCR instruments, there are slight differences in fluorescence signals between the PCR wells of these instruments, so reference Dye needs to be added for correction. This series of products is equipped with different concentrations of ROX Reference Dye, please choose according to the specific model.




1. Suitable for multiple system reactions.

2. Ability to predict and optimize reaction conditions in advance.



Descriptions

Possible Causes

Solution

Amplification curve is chaotic or  missing.

 

Incorrect instrument settings.

Improper concentration of primers or templates.

Adjust the settings according to the instrument manual.

Adjust the concentration of primers and templates.

Improper PCR reaction conditions.


Reduce annealing temperature, and extend extension time, etc. For target fragments with high GC-content, the denaturation time can be appropriately extended.


Primers or templates have advanced structures.

Redesign the primers.

Poor sample purity.

Further purify the sample.

Poor repeatability of quantitative values.

Incorrect instrument settings.

Adjust the settings according to the instrument manual.

Poor sample purity.

Further purify the sample.

Improper concentration of primers.

Adjust the concentration of primers.

Improper PCR reaction condition.

Reduce annealing temperature, and extend extension time, etc.

Improper primer design.

Redesign the primers

Experimental operation error.

Strictly follow the operating procedures to ensure accurate volume of each component in the reaction system.

Signal appears in the blank control.

Pollution of reaction system.


Replace the water used in the blank control. If the same situation persists, continue to replace the primers, pipette tips, PCR tubes, or use a new mix reaction system(prepare in the ultra clean bench to reduce aerosol pollution).



Probe method qua...

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