Modification Enzyme - Fast T4 DNA Ligase
Modification Enzyme - Fast T4 DNA Ligase

Modification Enzyme - Fast T4 DNA Ligase

T4 DNA Ligase (Fast) is produced by E. coli carrying Escherichia virus T4 gene. The enzyme catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.


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Article No.Product NameChinese NameSpecificationsPrice（Yuan）
DE00801ST4 DNA Ligase (Fast)快速 T4  DNA连接酶5 U/μl，1000 U488
DE00801MT4 DNA Ligase (Fast)快速 T4  DNA连接酶5 U/μl，5000 U2418

T4 DNA Ligase (Fast) is produced by E. coli carrying Escherichia virus T4 gene. The enzyme catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either sticky or blunt ends, but has no activity on single-stranded nucleic acids. Cloning of restriction enzyme generated DNA fragments, Site-directed mutagenesis, Cloning of PCR products, Nick repair in duplex DNA, RNA or DNA/RNA hybrids and Self-circularization of linear DNA. The enzyme requires ATP as a cofactor. sticky-end ligation is completed in 10 minutes at room temperature.


The enzyme joins DNA fragments with either sticky or blunt ends and repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.



1. Enzymes are strongly inhibited in NaCl or KCl concentrations higher than 200 mM

2. The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process. It is not recommended to add excessive T4 DNA Ligase (Fast) to the system

3. Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, the enzyme can be inactivated by heating before sample loading, and an appropriate amount of SDS can be added if necessary

4. PEG can greatly improve the ligation efficiency of blunt-ends, and the recommended addition amount of PEG8000 is 5% of the ligation system

5. The efficiency of electroconversion may be improved by removing T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction before prior to electro-transformation.

6. The number of transformants can be increased by extending the reaction time to 1 hour




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