PCR series - hot start taq DNA polymerase
PCR series - hot start taq DNA polymerase

PCR series - hot start taq DNA polymerase

This enzyme is HotStart Taq DNA polymerase and requires heating at 95 ° C for 5 minutes or 98 ° C for 2 minutes to activate. Taq DNA polymerase is a thermostable DNA polymerase derived from Thermophilic bacteria Thermous aquaticus recombined and expressed in E. coli, with a molecular weight of 94 kDa.

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Article NumberProduct NameChinese NameSpecificationsCatalog Price (Yuan)
DE00201SHS-Taq DNA polymeraseHot start taq DNA polymerase100U

128

DE00201MHS-Taq DNA polymeraseHot start taq DNA polymerase500U500


This enzyme is HotStart Taq DNA polymerase and requires heating at 95 ° C for 5 minutes or 98 ° C for 2 minutes to activate. Taq DNA polymerase is a thermostable DNA polymerase derived from Thermophilic bacteria Thermous aquaticus recombined and expressed in E. coli, with a molecular weight of 94 kDa. This enzyme has been specially modified to have strong amplification ability and extension speed. The length of the amplified fragment can reach 5-6kb, and the extension speed is 2-3kb/min (72 ℃). The enzyme has 5 '→ 3' polymerase activity and weak 5 '→ 3' Exonuclease activity; No 3 '→ 5' Exonuclease activity. The amplified product has a 3 '- dA tail.




1. This enzyme is a hot start polymerase that can improve the specificity of amplification and reduce non-specific amplification and primer dimerization;

2. After special modification, it has strong amplification ability and can effectively amplify 3-5 kb of DNA fragments under 2 kb/min extension conditions;

3. The thermal stability of the enzyme has been improved to a certain extent, which can be pre denatured at 98 ° C for 2 minutes;

4. Two step PCR can be performed at 60-72 degrees Celsius to shorten the entire PCR time;

5. The PCR product has a 3 '- dA tail and can be directly used for T/A cloning;

6. dUTP, dITP, and fluorescent labeled nucleotides can be incorporated.


1. This enzyme is a hot start TaqDNA polymerase that requires heating at 95 ° C for 5 minutes or 98 ° C for 2 minutes to activate; Therefore, it is beneficial to improve the specificity of amplification, reduce non-specific amplification and primer dimerization, and obtain good PCR results.

2. TaqDNA polymerase has deoxynucleotidyltransferase activity, so an extra Adenine is usually added to the 3 'end of the PCR product.

For non hot start enzymes, it is recommended to prepare a PCR reaction solution on ice and then place it in a PCR instrument for amplification. This is beneficial for improving the specificity of amplification, reducing non-specific amplification, and obtaining good PCR results.

4. Base error rate refers to the number of incorrect nucleotides incorporated into each base synthesis process. The base error rate of TaqDNA polymerase is 1 × 10-5.

5. If after PCR amplification, in addition to the target band, there are also other miscellaneous bands, it is recommended to increase the annealing temperature or perform gradient annealing to determine the optimal annealing temperature; At the same time, the amount of Taq enzyme in the system can be appropriately reduced to increase the specificity of PCR amplification reaction.

6. Our company's buffer has been optimized through a large number of PCR reaction examples, but there is an optimal magnesium ion concentration for certain PCR reactions. If you need to optimize the concentration of magnesium ions, please purchase our company's buffer and MgCl2/MgSO4 that do not contain magnesium ions.

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