PCR raw material enzyme
PCR raw material enzyme

PCR raw material enzyme

Polymerase chain reaction (PCR) is a molecular biological technology used to amplify specific DNA fragments. It is regarded as DNA replication in vitro, which can amplify a small amount of DNA fragments to a large extent, so as to achieve the degree of easy identification and recognition.

For PCR reactions, 'enzymes' play a crucial role, and each type of 'enzyme' has different activities, functions, and applications.

  • Product Introduction
  • Product Features
  • FAQ/Experimental Cases
  • Manual Download


Raw enzyme


Product NameArticle NumberRecommended Use
Taq DNA PolymeraseDAP07P01Qualitative and quantitative PCR

Antibody modified hot start Taq DNA polymerase

Chemically modified hot start Taq DNA polymeraseDAP07P03qPCR、PCR

Reverse transcriptase M-MLV

DAP07P04cDNA synthesis、RT-qPCR、RT-RPA
Murine RNase inhibitorDAP07P05cDNA synthesis、RT-qPCR、RT-RPA

Multiple amplifications

PCR polymerase technology mainly includes: ① high-temperature denaturation: promoting the dissociation of the extracted original DNA double stranded (template) and the amplified double stranded DNA at a temperature of 94 ℃, forming a single strand, preparing for subsequent amplification; ② Low temperature annealing renaturation: At 55 ℃, the unraveled DNA single strand binds to upstream and downstream primers; ③ Thermophilic extension: DNA template primer conjugate, under the action of Taq DNA polymerase, takes dNTP as raw material and continues to extend along the primer to generate a replication chain paired with template DNA. These three steps go round and round, and the new DNA strand generated in the previous cycle becomes the template for the next cycle, which can amplify the target gene by hundreds of thousands or even millions of times in a short period of time.

Chemically modif...

File Name:

Antibody modifie...

File Name:

DAP07P01- Taq DN...

File Name:

DAP07P04- Instru...

File Name:

DAP07P05-RNA enz...

File Name:

Related Recommend