1. Endonuclease should be set in an ice box when used, and should be stored at -20 ℃ immediately after use.
2. If the expected cleavage site cannot be cleaved, please confirm if there is a methylation interference issue.
3. For research use only. Not for use in diagnostic procedures and food or medicine. Not allowed to be stored in ordinary residential areas.
4. For your safety and health, please wear White coat and disposable gloves.
Special note: The recommended incubation temperature for this SmaI is 30 ℃, with 50% enzyme activity at 37 ℃ and 100% enzyme activity at 25 ℃.