Modification enzyme —Endonuclease IV
Modification enzyme —Endonuclease IV

Modification enzyme —Endonuclease IV

Endonuclease IV produced by our company is derived from E.coli. It is a nucleic acid endonuclease, also called Nfo, which can participate in several oxidative damages on DNA molecules.


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Article No.Product NameChinese NameSpecificationsPrice（Yuan）
DE01101SEndonuclease IV (Nfo)核酸内切酶IV1000U1000
DE01101MEndonuclease IV (Nfo)核酸内切酶IV5000U4000

Endonuclease IV produced by our company is derived from E.coli. It is a nucleic acid endonuclease, also called Nfo, which can participate in several oxidative damages on DNA molecules. The enzyme can recognize the depurine/depyrimidine (AP) site on the double stranded DNA molecule and cut the first Phosphodiester bond at the 5´ end of the AP site to produce 3´ hydroxyl and 5´ Deoxyribose phosphate terminal; In addition, the enzyme also has 3´ diesterase activity, which can release Glyceraldehyde phosphate, complete Deoxyribose 5´ - phosphate and phosphate from the 3´ end of DNA; At the same time, the enzyme also has 3´ -5´ Exonuclease activity.






1. Single cell gel electrophoresis (comet test), recommended dilution ratio of comet test 1:104~1:105

2. Alkali elution

3. Alkaline hydrolysis spin



1. The gradual action of this enzyme on the substrate is influenced by metal ions, DTT, EDTA, etc., and it tends to act more on the double stranded DNA at the 3´ concave end.

2. For your safety and health, please wear White coat and disposable gloves.



Nucleic acid End...

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