PCR Series-Pfu DNA Polymerase
PCR Series-Pfu DNA Polymerase

PCR Series-Pfu DNA Polymerase

Pfu DNA polymerase is a thermostable enzyme of approximately 90 kDa isolated from Pyrococcus furiosus. recombined and expressed in E. coli, with a molecular weight of 90kD. Its authenticity is about 18 times that of ordinary TaqDNA polymerase.


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Article No.Product NameChinese NameSpecificationsPrice（Yuan）
DE00401SPfu DNA polymerasePfu DNA聚合酶100U128
DE00401MPfu DNA polymerasePfu DNA聚合酶500U460

Pfu DNA polymerase is a thermostable enzyme of approximately 90 kDa isolated from Pyrococcus furiosus. recombined and expressed in E. coli, with a molecular weight of 90kD. Its authenticity is about 18 times that of ordinary TaqDNA polymerase. This enzyme has been specially modified to have strong amplification ability and extension speed. The length of the amplification fragment can reach 5-6kb, and the extension speed is 1 kb/min(72℃). The enzyme has 5 '-3' polymerase activity and 3 '-5' exonuclease activity. Pfu DNA polymerase-generated PCR fragments are blunt-ended, so it is not suitable for TA cloning.


1. This enzyme has undergone special modifications and has enhanced amplification ability and extension speed compared with traditional enzymes. It can effectively amplify 5-6kb of DNA fragments under the condition that the extension speed is 1kb/min (72 ℃).

2. Good thermal stability: the half-life at 98 ℃ exceeds 40 minutes.

3. Lowest error rate.

4. Not tolerant to dUTP, dITP.

5. PCR products have blunt-ends and can be directly used for blunt cloning.


1. Pfu DNA polymerase has a strong 3 '-5' Exonuclease activity, and the PCR products are blunt-ended, so it is not suitable for T/A cloning. The products need to be added deoxyadenosine (A) with using Taq DNA polymerase before using for TA cloning.

2. It is recommended to prepare PCR reaction solution on ice and then place it in a PCR instrument for amplification. This is beneficial for improving the specificity of amplification, reducing non-specific amplification, and obtaining good PCR results.

3. If there are other heterobands in addition to the target band after PCR amplification, it is recommended to appropriately reduce the enzyme dosage in the system to increase the specificity of the PCR amplification reaction.

4. Our company's buffer has been optimized through a large number of PCR examples, but there is an optimal magnesium ion concentration for certain PCR. If you need to optimize the concentration of magnesium ions, please purchase a buffer from our company that does not contain magnesium ions and MgCl2/MgSO4.

5. dUTP, dITP and primers containing these nucleotides cannot be used for polymerase catalyzed PCR amplification as the enzyme will inhibit DNA polymerization when combined with DNA template containing Uracil and Hypoxanthine


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