What are the raw materials for fluorescence immunochromatography reagents?

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The emergence of fluorescent immunochromatographic reagents has certain benefits for people, and can help them conduct testing. Today, let's take a look at what constitutes the raw materials of fluorescent immunochromatographic reagents, so that everyone can have a grassroots understanding and help those in need know the ingredients.

  What are the raw materials for fluorescence immunochromatography reagents?

(1) Fluorescent pigment

1. Fluorescein isothiocyanate (FITC): it displays bright yellow green fluorescence.

2. Tetraethyl Rhodamine (RB200): orange red fluorescence.

3. Tetramethyl Rhodamine Isothiocyanate (TRITC): orange red fluorescence.

4. Phycoerythrin (R-RE): Has bright orange fluorescence.

(2) Other fluorescent substances

1. Lanthanide Chelation: Eu3 is widely used. Eu3 Chelation has wide excitation wavelength range, narrow emission wavelength range and long fluorescence attenuation time, which is suitable for time-resolved fluorescence Immunoassay.

2. The substance that generates fluorescence after enzyme action: 4-methylfimbrionone -- D-galactoside itself does not have fluorescence, and is decomposed into 4-methylfimbrionone by galactosidase, which can emit fluorescence. Other substrates such as Alkaline phosphatase are 4-methyl umbelliferone phosphate. The substrate of Horseradish peroxidase is p-hydroxy Phenylacetic acid, etc.

Fluorescence chromatography is a novel membrane detection technique based on antigen antibody specific immune reactions. This technology uses detection lines (coated antibodies or coated antigens) and quality control lines (anti antibodies) as stationary phases, test solution as mobile phase, fluorescent labeled antibodies or antigens are fixed on connecting pads, and the analyte to be analyzed is moved on the chromatographic plate through capillary action. This is its usage process, where the two are combined to measure.

From the above content, there is also a clear understanding of the raw materials for fluorescent immunochromatography reagents. Many of these materials have certain proprietary terms, so when we further understand them, those who do not specialize in this aspect are not very familiar with the overall situation. Therefore, if there is a need and a desire to know the ingredients inside, they need to consult them one by one, I hope the above content can effectively help everyone.

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