Gene synthesis refers to the technology of artificially synthesizing double stranded DNA molecules in vitro, which can be divided into enzymatic synthesis and chemical synthesis methods. It is a technology that synthesizes genes using artificial methods, and gene synthesis services do not require templates, so they are not limited by gene sources.

Advantages of gene synthesis services:

One is to ensure the complete correctness of the sequence and short synthesis cycle;

Secondly, it is possible to modify the enzyme cleavage sites and gene sequences of genes, with great flexibility;

Thirdly, experimenters can design genes that are difficult to obtain or even non-existent in nature according to their own preferences;

The fourth is the ability to optimize codons.

Methods for gene synthesis services:

1. Solid-phase synthesis: oligonucleotide synthesis is achieved by adding nucleotide monomers one by one at the end of the synthetic chain. The synthesis of each oligonucleotide involves four steps: deprotection, coupling, blocking, and oxidation.

2. Chip DNA synthesis: The technology of synthesizing DNA using a series of electrochemical techniques using microarray chips. It is cheaper than Solid-phase synthesis, and can produce a larger number of target genes, but the accuracy of this method is not comparable to it.

3. PCR synthesis: The gene fragments generated by the PCR method can be completed through various cellular systems. For example, using a yeast system, different genes can be added to yeast chromosomes by labeling enzymes and labels with different incisions.

1、Application scenarios for gene synthesis services:

2、Gene optimization to improve gene expression

3、Synthesis of difficult to amplify genes with known sequences

4、Synthesize a large amount of cDNA for microchips

5、Human cloning mouse antibody or recombinant antibody

6、Design and synthesis of gene vaccines and gene therapy vectors

7、Modify and design genes based on the structural and functional research of genes

8、Synthesize different gene mutant strains, SNPS, or other mutant strains

Gene synthesis has applications in many fields, including from gene loops to improved metabolism and more. With the advancement of technology, scientists are able to modify and design genes. When researchers insert a synthetic gene fragment with a length exceeding 100kbp into a host bacteria that lacks its own genetic material, the bacteria can successfully produce new cells and even be used to construct new metabolic systems in living cells. Therefore, it also indicates that gene synthesis has great potential in fields such as medicine.