DSL

desalination 

5-45 nt

Can remove most of the salt generated during synthesis and ammonolysis processes

first generation sequencing, regular PCR, multiple PCR, whole gene synthesis, experiments with low purity requirements, etc.

70%-85%

46-85 nt

regular PCR, whole gene synthesis, experiments with low purity requirements, etc.

45%-70%

OPC/iPAGE

Column purification

15-50 nt

Can remove most of the salt and some small fragments

first generation sequencing, regular PCR, multiple PCR, whole gene synthesis, subcloning, site mutation, experiments with general purity requirements, etc.

85%-90%

PAGE

（polyacrylamide gel electrophoresis ）

15-120 nt

Can remove most small fragments and salts

first generation sequencing, regular PCR, multiple PCR, whole gene synthesis, subcloning, site mutation, RT-PCR, complementary primer annealing, mass spectrometry detection, gene construction, antisense nucleic acid, NGS primer, partially modified primer, experiments with high purity requirements, etc.

85%-95%

HPLC

(Inverted C18 or ion exchange)

High performance liquid chromatography

5-60 nt

Can remove most small fragments and salts

first generation sequencing, regular PCR, multiple PCR, whole gene synthesis, subcloning, site mutation, RT-PCR, complementary primer annealing, mass spectrometry detection, gene construction, antisense nucleic acid, NGS primer, all modified primers, diagnostic reagent raw materials, experiments requiring high purity, etc.

90%-99.9%

Various purification methods mentioned above

Combination of methods

5-120 nt

The most extreme purification method to remove almost all impurities and salts

High level diagnostic reagent raw materials, experiments with extremely high purity requirements, etc.

95%-99.9%