BirA enzyme (biotin ligase)
BirA enzyme (biotin ligase)

BirA enzyme (biotin ligase)

DIA-UP BirA enzyme (biotin ligase) specifically recognizes defined protein tags and catalyzes site-specific covalent conjugation between biotin and target proteins to achieve exclusive oriented biotinylation. Biotin binds firmly to fixed protein sites without disrupting native protein structure or bioactivity, delivering highly homogeneous, stable labeling that boosts IVD assay sensitivity, specificity, repeatability and batch consistency. Compared with traditional NHS ester chemical labeling, BirA enzymatic labeling generates negligible background interference and eliminates tedious purification, greatly elevating manufacturing efficiency and product stability. 

  • Product Introduction
  • Product Features
  • FAQ/Experimental Cases
  • Manual Download

Ditch Random Disordered Modification

BirA Enzymatic Site-Specific Labeling Delivers Controllable Biotinylation for IVD

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BirA enzyme (biotin ligase)



Newly Launched DIA-UP BirA Enzyme

Precise Catalysis, Oriented Site Labeling

DIA-UP BirA enzyme (biotin ligase) specifically recognizes defined protein tags and catalyzes site-specific covalent conjugation between biotin and target proteins to achieve exclusive oriented biotinylation. Biotin binds firmly to fixed protein sites without disrupting native protein structure or bioactivity, delivering highly homogeneous, stable labeling that boosts IVD assay sensitivity, specificity, repeatability and batch consistency. Compared with traditional NHS ester chemical labeling, BirA enzymatic labeling generates negligible background interference and eliminates tedious purification, greatly elevating manufacturing efficiency and product stability.


01

DIA-UP BIOTECH

- Preserve Bioactivity -


Labeling only at fixed sites to fully retain protein function and activity.


02

DIA-UP BIOTECH

- Lock Long-Term Stability -


Covalent linkage prevents biotin shedding, tolerates harsh reaction conditions and extends reagent shelf life.


03

DIA-UP BIOTECH

- Control Homogeneity -


Uniform labeling site & count on every protein for minimal inter-batch deviation and consistent assay results.


04

DIA-UP BIOTECH

- Boost Assay Performance -


Amplify target signals, cut background noise and improve IVD sensitivity & specificity.




DIA-UP BirA enzyme (biotin ligase)


Catalog No.: H001P33

Source: Recombinant

Appearance: Clear colorless liquid

Buffer: Tris buffer, pH 8.0

Purity: ≥70% via SDS-PAGE

Filtration: 0.22 μm sterile filtered

Application: Biotin labeling of AVI-tagged proteins

Storage: Store long-term below -20°C; avoid repeated freeze-thaw cycles

Notes: Optimal BirA concentration varies with assay conditions; determine working concentration via pre-experiments.


Reaction System (100 μL total volume as example):

BirA enzyme (biotin ligase)

∠ Top up to 100 μL with deionized water, mix well, and incubate in a 30°C water bath for 30–40 min.
∠ Remove free biotin via ultrafiltration or dialysis; a dilution ratio above 1000-fold is recommended.

∠ The optimal final concentration of the protein to be biotinylated in the reaction system ranges from 40 μM to 80 μM:

  • If final concentration < 40 μM, extend incubation time to 8 h or longer;

  • If final concentration is above 40 μM, proportionally increase the dosage of BirA enzyme and biotin;

  • If final concentration > 80 μM, dilute the protein before reaction.



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