1.快速内切酶,可在 5~15min 内完成反应
2.最适反应温度为 37℃
3.受 EcoBI 甲基化影响,序列可能重叠,剪切受阻
4.失活条件为 80℃温育 20min
5.3h 温育未表现星号活性,延时酶切可能出现星号活性
1. Rapid: the reaction can be completed in 5~15 min.
2. 37 ℃ is the most suitable reaction temperature.
3. Affected by EcoBl methylation, sequences may overlap and digestion blocking.
4. Enzyme inactivation after 20 minutes of incubation at 80 ℃.
5. 3-hour incubation did not show star activity, and delayed enzyme digestion may show star activity.