1.快速内切酶,可在5~15min内完成反应
2.最适反应温度为37℃
3.不可热失活
4.3h温育未表现星号活性,延时酶切可能出现星号活性
1. Rapid: the reaction can be completed in 5~15 min.
2. 37 ℃ is the most suitable reaction temperature.
3. Non thermal deactivation.
4. 3-hour incubation did not show star activity, and delayed enzyme digestion may show star activity.